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Flow Cytometric Determinations of Glycogen Content of Yeast During Fermentation
Author(s) -
Hutter K.J.
Publication year - 2002
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.2002.tb00123.x
Subject(s) - yeast , glycogen , flow cytometry , fermentation , stain , biochemistry , biology , chemistry , staining , microbiology and biotechnology , genetics
Using flow cytometric analysis, the rapid detection of glycogen content of yeast cells in process is possible. Glycogen is a sensitive parameter which can express the physiological state of the yeast cells. A procedure was developed to stain and measure glycogen in yeast cells using flow cytometry. Glycogen content of lager yeast cells was tested after 1 and 15 h of nutrient limitation.