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TRANSFER OF NUCLEAR GENES DURING CYTODUCTION IN SACCHAROMYCES
Author(s) -
Goodey A. R.,
Brown A. J. P.,
Tubb R. S.
Publication year - 1981
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.1981.tb04023.x
Subject(s) - nuclear gene , chromosome , genetics , hybrid , gene , biology , saccharomyces cerevisiae , yeast , saccharomyces , transfer rna , genome , botany , rna
Transfer of a nuclear gene STA 3 was obtained as a rare‐event when forcibly selected for during cytoduction. A stable hybrid which inherited STA 3 in this way also received the markers MAT a and HIS 4 from chromosome III. Genetic analyses showed that the transferred nuclear genes remained chromosomally located and were not inherited cytoplasmically; the hybrid, therefore, was disomic at least for chromosome III. The technique described here can be used to construct yeast hybrids which receive only a discrete portion of nuclear material from one parent.