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The use of Random Amplified Polymorphic DNA (RAPD) markers for the study of taxonomical relationships among species of ASPHODELUS sect. Verinea (Asphodelaceae)
Author(s) -
Lifante Zoila Díaz,
Aguinagalde Itzi'ar
Publication year - 1996
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1996.tb12788.x
Subject(s) - rapd , biology , botany , taxon , primer (cosmetics) , population , genetic diversity , chemistry , demography , organic chemistry , sociology
RAPD analysis was applied to the three species of Asphodelus sect. Verinea (Pomel) Boissier (Asphodelaceae): Asphodelus fistulosus L., A. ayardii Jahand. & Maire, and A. tenuifolius Cav. Fifteen populations, five per species, were used and eight primers were sampled. A total of 97 amplification products were generated, and 4–12 polymorphic products per primer were obtained. Several specific RAPD markers were detected for A. ayardii and A. tenuifolius , while only two for A. fistulosus , which shares most amplification products with the two former species. Results that reinforce the specific status of the three taxa are shown. Asphodelus tenuifolius showed the highest interpopulation variability in agreement with the high specialization of other characters in this species. In accordance with these results, an amphidiploid origin for A. fistulosus , with the participation of A. ayardii and A. tenuifolius , is suggested.