Premium
Kin recognition between medial Golgi enzymes in HeLa cells.
Author(s) -
Nilsson T.,
Hoe M.H.,
Slusarewicz P.,
Rabouille C.,
Watson R.,
Hunte F.,
Watzele G.,
Berger E.G.,
Warren G.
Publication year - 1994
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1994.tb06294.x
Subject(s) - watson , biology , library science , computer science , artificial intelligence
The medial Golgi enzymes, N‐acetylglucosaminyltransferase I (NAGT I) and mannosidase II (Mann II), and the trans Golgi enzyme, beta‐1,4‐galactosyltransferase (GalT) were each retained in the endoplasmic reticulum (ER) by grafting on the cytoplasmic tail of the p33 invariant chain. Transient and stable expression of p33/NAGT I in HeLa cells caused relocation of endogenous Mann II to the ER and transient expression of p33/Mann II had a similar effect on endogenous NAGT I. Neither of these endogenous medial enzymes were affected by transient expression of p33/GalT. These data provide strong evidence for kin recognition between medial Golgi enzymes and suggest a role for them in the organization of the Golgi stack.