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The human muscle nicotinic acetylcholine receptor alpha‐subunit exist as two isoforms: a novel exon.
Author(s) -
Beeson D.,
Morris A.,
Vincent A.,
NewsomDavis J.
Publication year - 1990
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1990.tb07378.x
Subject(s) - biology , nicotinic acetylcholine receptor , gene isoform , acetylcholine receptor , exon , alpha (finance) , interleukin 10 receptor, alpha subunit , g alpha subunit , interleukin 5 receptor alpha subunit , protein subunit , ganglion type nicotinic receptor , nicotinic agonist , receptor , microbiology and biotechnology , genetics , gene , medicine , construct validity , nursing , patient satisfaction
Analysis of acetylcholine receptor clones isolated from a human leg muscle cDNA library, revealed that the alpha‐subunit existed as two isoforms. A novel exon, coding for 25 amino acids, was located in the human genomic DNA sequence; its insertion into the alpha‐subunit gives the new isoform of 462 amino acids. In addition, mRNAs for the two isoforms were found in equal proportions in poly(A)+ RNA obtained from three further sources including partially denervated and innervated human muscle and the rhabdomyosarcoma cell line TE671. Both protein isoforms can be expressed in E. coli. No evidence of a sequence related to that of the new exon was found in cDNA derived from poly(A)+ RNA isolated from fetal calf or embryonic chick muscle or Torpedo marmorata electric organ.