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Properties of a monoclonal antibody to epidermal growth factor receptor with implications for the mechanism of action of EGF.
Author(s) -
Gregorou M.,
Rees A.R.
Publication year - 1984
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1984.tb01910.x
Subject(s) - biology , epidermal growth factor , monoclonal antibody , epidermal growth factor receptor , mechanism (biology) , mechanism of action , receptor , microbiology and biotechnology , antibody , immunology , genetics , in vitro , philosophy , epistemology
A monoclonal antibody, EGR/ G49, raised against the receptor for epidermal growth factor (EGF) present in A431 cells inhibits EGF binding by decreasing the affinity of the major population of low affinity receptors while leaving the minor high affinity population relatively unperturbed. The antibody, which binds to a carbohydrate determinant at a site distinct from the EGF binding site, induces clustering and internalisation of the receptor without stimulating the EGF receptor‐kinase or affecting its ability to undergo stimulation by EGF. It is toxic to A431 cells and induces morphological changes similar to those seen when these cells are challenged with EGF in the concentration range 1‐10 nM. These results suggest that high and low affinity EGF receptors can be distinguished and that they may serve different functions.