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Multiplicity‐dependent biological and biochemical properties of epstein‐barr virus (EBV) rescued from non‐producer lines after superinfection with P3HR‐1 EBV
Author(s) -
Cho MyungSam,
Fresen KarlOtto,
Hausen Harald Zur
Publication year - 1980
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910260316
Subject(s) - superinfection , biology , virology , epstein–barr virus , virus , genome , multiplicity of infection , recombinant dna , dna , microbiology and biotechnology , gene , genetics
Abstract Superinfection of lymphoblastoid cells of EBV non‐producer lines with non‐transforming P3HR‐l EBV leads to the rescue of transforming virus. At least part of the recovered molecules represent recombinant DNA between superinfecting P3HR‐l EBV and resident viral genomes (Fresen et al., 1979, 1980). With high titer stocks of superinfecting P3HR‐l EBV, viral particles with early antigen (EA)‐inducing properties can be rescued, indicating that under these conditions of infection input viral genomes may become replicated. Sequential blot analysis with 32 P‐P3HR‐l EBV DNA of intracellular viral DNA synthesized following infection reveals a multiplicity‐dependent pattern. High particle inputs lead to preferential synthesis of certain fragments, independent of infection of either EBV genome‐free or EBV genome carrier cells. This accumulation of specific viral sequences indicates defective replication of P3HR‐l EBV DNA.