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Cloning and sequencing of a t(14;19) breakpoint that involves the Cμ switch region
Author(s) -
Crossen Peter E.,
Kennedy Martin A.,
Heaton David C.,
Morrison Mary J.
Publication year - 1993
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.2870080110
Subject(s) - breakpoint , chromosomal translocation , genetics , biology , chromosome , microbiology and biotechnology , chromosome 22 , gene , breakpoint cluster region
Abstract The t(14;19) is a recurring translocation found in a small number of cases of chronic B‐cell leukemia (CLL). We have cloned and sequenced the breakpoint in a patient with a t(14;19) and shown that the breakpoint on chromosome 14 occurred in the Cμ switch region, and that the breakpoint on chromosome 19 occurred in the 5′ untranslated region of the BCL 3 gene. This is in contrast to all the other reported cases with a t(14;19) in which the breakpoints on chromosome 14 occurred in the Cαl or Cα2 switch region, and the breakpoints on chromosome 19 occurred upstream of the BCL 3 gene. Our results further emphasize the importance of the switch region in the t(14;19) translocation. © 1993 Wiley‐Liss, Inc.