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Suppressive subtractive hybridization libraries prepared from the digestive gland of the oyster Crassostrea brasiliana exposed to a diesel fuel water‐accommodated fraction
Author(s) -
Lüchmann Karim Hahn,
Mattos Jacó Joaquim,
Siebert Marília Nardelli,
Dorrington Tarquin Stephen,
ToledoSilva Guilherme,
Stoco Patricia Hermes,
Grisard Edmundo Carlos,
Bainy Afonso Celso Dias
Publication year - 2012
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.1837
Subject(s) - suppression subtractive hybridization , diesel fuel , oyster , biology , marine invertebrates , crassostrea , gene , innate immune system , transcriptome , immune system , fishery , chemistry , biochemistry , gene expression , immunology , cdna library , organic chemistry
Abstract Diesel fuel can cause adverse effects in marine invertebrates by mechanisms that are not clearly understood. The authors used suppressive subtractive hybridization to identify genes up‐ and downregulated in Crassostrea brasiliana exposed to diesel fuel. Genes putatively involved in protein regulation, innate immune, and stress response, were altered by diesel challenge. Three genes regulated by diesel were validated by quantitative real‐time polymerase chain reaction. This study sheds light on transcriptomic responses of oysters to diesel pollution. Environ. Toxicol. Chem. 2012;31:1249–1253. © 2012 SETAC