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Phenylalanine functionalized zwitterionic monolith for hydrophobic interaction electrochromatography
Author(s) -
Wang Jiabin,
Jia Wenchao,
Lin Xucong,
Wu Xiaoping,
Xie Zenghong
Publication year - 2013
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201300367
Subject(s) - monolith , capillary electrochromatography , glycidyl methacrylate , chemistry , methacrylate , monolithic hplc column , polymer , chromatography , electrochromatography , divinylbenzene , chemical engineering , organic chemistry , monomer , copolymer , catalysis , high performance liquid chromatography , capillary electrophoresis , styrene , engineering
A novel phenylalanine (Phe) functionalized zwitterionic monolith for hydrophobic electrochromatography was prepared by a two‐step procedure involving the synthesis of glycidyl methacrylate based polymer monolith and subsequent on‐column chemical modification with Phe via ring‐opening reaction of epoxides. Benefitting from the hydrophobicity of both methacrylate‐based matrix and aromatic group of Phe, this monolith could exhibit good hydrophobic interaction for the separation. Typical RP chromatographic behavior was observed toward various solutes. The well‐controlled cathodic or anodic EOF of the prepared column could be facilely switched by altering the pH values of running buffers. The separation mechanism of this Phe functionalized zwitterionic monolith is discussed in detail. Two mixed‐mode mechanisms of RP/cation exchange and RP/anion exchange could be further realized on the same monolith in different pH condition of the mobile phase. Versatile separation capabilities of neutral, basic, and acidic analytes have been successfully achieved in this zwitterionic monolith by CEC method.