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Genomic analysis of S accharomyces cerevisiae isolates that grow optimally with glucose as the sole carbon source
Author(s) -
Aragon Anthony D.,
TorrezMartinez Norah,
Edwards Jeremy S.
Publication year - 2012
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201200172
Subject(s) - biology , genetics , gene , nonsynonymous substitution , population , genetic analysis , strain (injury) , gene expression , single nucleotide polymorphism , genotype , genome , demography , anatomy , sociology
A population of S accharomyces cerevisiae was cultured for approximately 450 generations in the presence of high glucose to select for genetic variants that grew optimally under these conditions. Using the parental strain BY 4741 as the starting population, an evolved culture was obtained after aerobic growth in a high glucose medium for approximately 450 generations. After the evolution period, three single colony isolates were selected for analysis. Next‐generation Ion Torrent sequencing was used to evaluate genetic changes. Greater than 100 deletion/insertion changes were found with approximately half of these effecting genes. Additionally, over 180 SNP s were identified with more than one‐quarter of these resulting in a nonsynonymous mutation. Affymetrix DNA microarrays and RN seq analysis were used to determine differences in gene expression in the evolved strains compared to the parental strain. It was established that approximately 900 genes demonstrated significantly altered expression in the evolved strains relative to the parental strain. Many of these genes showed similar alterations in their expression in all three evolved strains. Interestingly, genes with altered expression in the three evolved strains included genes with a role in oxidative metabolism. Overall these results are consistent with the physiological observations of optimal growth with glucose as the carbon source. Namely, the decreased ethanol production suggest that the underlying metabolism switched from fermentation to respiration during the selection for optimal growth on glucose.