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Chiral analysis of amphetamine‐type stimulants using reversed‐polarity capillary electrophoresis/positive ion electrospray ionization tandem mass spectrometry
Author(s) -
Iwata Yuko T.,
Kanamori Tatsuyuki,
Ohmae Yoshihito,
Tsujikawa Kenji,
Inoue Hiroyuki,
Kishi Tohru
Publication year - 2003
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305431
Subject(s) - chemistry , ephedrine , chromatography , pseudoephedrine , electrospray ionization , capillary electrophoresis , tandem mass spectrometry , mass spectrometry , electrospray , mass spectrum , analytical chemistry (journal) , neuroscience , biology
Abstract Reversed‐polarity (RP) capillary electrophoresis/positive ion electrospray ionization mass spectrometry (CE‐ESI+ MS) and tandem mass spectrometry (MS/MS) were utilized for simultaneous chiral separation of nine amphetamine‐type stimulants (ATS) ( dl ‐norephedrine, dl ‐norpseudoephedrine, dl ‐ephedrine, dl ‐pseudoephedrine, dl ‐amphetamine, dl ‐methamphetamine, dl‐ methylenedioxyamphetamine, dl‐ methylenedioxymethamphetamine, and dl‐ methylenedioxyethylamphetamine). Using highly sulfated γ‐cyclodextrin (SU(XIII)‐γ‐CD) as a chiral selector, the nine ATS were completely separated within 50 min. The migrated ATS‐CD complex was dissociated at the ESI interface, and only ATS molecules went into the MS detector so that all 18 individual enantiomers were identified by their mass spectra. The detection limit of MS/MS was 10 times more sensitive than those for single MS. Seized d ‐methamphetamine hydrochloride samples dissolved at high concentration (20 mg/mL) were analyzed. Impurities originating in the precursor such as l ‐ephedrine and d ‐pseudoephedrine were detected and identified by tandem mass spectra.