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Transverse pore gradient gel electrophoresis, using the PhastSystem
Author(s) -
Buzás Zsuzsanna,
Wheeler David L.,
Garner Mark M.,
Tietz Dietmar,
Chrambach Andreas
Publication year - 1994
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501501153
Subject(s) - electrophoresis , transverse plane , chromatography , concentration gradient , materials science , gel electrophoresis , temperature gradient gel electrophoresis , chemistry , analytical chemistry (journal) , anatomy , medicine , biochemistry , 16s ribosomal rna , gene
Abstract The application of pore gradient gels prefabricated for the PhastSystem (Pharmacia) to transverse pore gradient gel electrophoresis is demonstrated. It has the twofold advantage of (i) horizontal positioning, avoiding gel stretching during the preparation of these gels and resulting pore size irreproducibility experienced with vertically applied pore gradient gels, which necessitate an orthogonal transfer of spacers, and (ii) miniaturized gel dimensions, which allow a small sample load and a short duration of electrophoresis and staining.