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Improved rehydration by controlled drying of polyacrylamide gels for hybrid isoelectric focusing
Author(s) -
Rossmann Ute,
Altland Klaus
Publication year - 1987
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150081209
Subject(s) - urea , isoelectric focusing , chromatography , chemistry , polyacrylamide , isoelectric point , immobilized ph gradient , polymer chemistry , organic chemistry , enzyme
Abstract A procedure is described to improve the properties of dried gels, containing immobilized pH gradients, for rehydration in the presence of urea. Triton X‐100, reducing agents and carrier ampholytes. When gels are dried by a fan in ambient atmosphere for the shortest time interval required to achieve an evenly flat surface, rehydration in the presence of 8 mol/L urea and 30 or 5 mL/L of Triton X‐100 is completed within 4 or 1 h, respectively. These rehydration properties can be maintained by storing the dried and sealed gels at −20 °C.