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Degradation kinetics of an alkaline Immobiline in the frozen state
Author(s) -
AstruaTestori Silvia,
Pernelle JeanJacques,
Wahrmann Juan P.,
Righetti Pier Giorgio
Publication year - 1986
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150071109
Subject(s) - kinetics , chemistry , hydrolysis , degradation (telecommunications) , alkaline hydrolysis , hydrolytic degradation , chromatography , nuclear chemistry , biochemistry , telecommunications , physics , quantum mechanics , computer science
Abstract The degradation kinetics of Immobiline p K 9.3 (0.2 m in H 2 O) have been studied over a period of 2‐years in the frozen state. The apparent hydrolysis rate (derived from the shift of myosin spots in a two‐dimensional map) is 20% per year (or 10% if incorporation of acrylic acid, an hydrolysis product, is accounted for). Such reactions in frozen solutions are not uncommon ( cf. the spontaneous mutarotation of glucose, Kiovsky, T. E. and Pincock, R. E., J. Amer. Chem. Soc. 1966, 88 , 4704–4710).