SECM for Studying the Immobilization and Repartition of a Redox Anti‐tetracycline Aptamer on Screen‐printed Carbon Electrodes

Scanning electrochemical microscopy (SECM) is discussed as a versatile tool to provide a unique approach of localized electrochemical information in the context of biosensing research. The step‐by‐step immobilization of DNA aptamer with intrinsic redox activity on screen‐printed carbon electrode (SPCE) was successfully monitored using SECM imaging tool. Control experiments were performed with a non‐electroactive aptamer. After immobilization of these aptamers, SECM images showed the repartition of the electroactive anti‐tetracycline aptamer when comparing with images produced for control and for all modification steps of SPCE. The possibility of tetracycline detection was also proved by causing a decrease in recorded current.