Premium
Substantial Influence of Temperature on Anchoring of Gold‐Nanoparticle Monolayer for Performance of DNA Biosensors
Author(s) -
Nowicka Anna M.,
Kowalczyk Agata,
Donten Mikolaj,
Leech Dónal,
Hepel Maria,
Stojek Zbigniew
Publication year - 2010
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201000241
Subject(s) - biosensor , monolayer , colloidal gold , nanotechnology , nanoparticle , detection limit , materials science , aptamer , layer (electronics) , dithiol , chemistry , self assembled monolayer , analytical chemistry (journal) , chromatography , organic chemistry , biology , genetics
Abstract This paper presents a way of modification of crystalline gold surface with a high quality layer of gold nanoparticles (Au NPs) via self‐assembled dithiol. The application of additional Au NPs monolayer prepared at various temperatures was tested with three types of biosensors previously described in the literature. The examined DNA biosensors differed by the detection method and the way of the immobilization of DNA probe at the modified gold electrode surface. For the immobilization of DNA probe in the sensing layer either the formation of SAM or the affinity binding (biotin – sterptavidin) or covalent attachment were used. The necessary condition of successful preparation of a perfect such monolayer is the preparation temperature of 4 °C. The preparation of Au NPs layers at higher than 4 °C temperatures leads to poor repeatability and unsatisfactory precision of the measurements. The application of the perfect Au monolayer lowers the detection limit (circa by 10 to 100 times) for all tested DNA biosensors.