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Derivative chronopotentiometric stripping analysis of insulin
Author(s) -
Honeychurch Michael J.,
Ridd Michael J.
Publication year - 1996
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140080111
Subject(s) - chemistry , hanging mercury drop electrode , stripping (fiber) , oxygen , potentiometric titration , mercury (programming language) , inorganic chemistry , electrode , chromatography , analytical chemistry (journal) , voltammetry , electrochemistry , organic chemistry , materials science , composite material , computer science , programming language
Abstract Porcine insulin was analyzed in the presence of dissolved oxygen by constant current stripping analysis (CCSA) and potentiometric stripping analysis (PSA). The CCSA analysis involves the constant current reduction of mercuric insulin thiolate following its potentiostatic accumulation on a hanging mercury drop electrode. The CCSA response was quantitatively enhanced in the presence of dissolved oxygen. The PSA analysis involves the oxidation, by dissolved oxygen, of sulfhydryl groups in reduced insulin following its accumulation on the HMDE at −900mV (vs. SCE).