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Tocopherols and tocotrienols in grape seeds from 14 cultivars grown in Korea
Author(s) -
Wie Minjung,
Sung Jeehye,
Choi Youngmin,
Kim Younghwa,
Jeong HeonSang,
Lee Junsoo
Publication year - 2009
Publication title -
european journal of lipid science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 94
eISSN - 1438-9312
pISSN - 1438-7697
DOI - 10.1002/ejlt.200900058
Subject(s) - tocopherol , tocotrienol , saponification , cultivar , chemistry , extraction (chemistry) , chromatography , horticulture , botany , vitamin e , food science , biology , antioxidant , biochemistry
Abstract In this study, the tocopherol (T) and tocotrienol (T3) contents of grape seeds from 14 different varieties grown in Korea were analyzed using saponification extraction followed by normal‐phase liquid chromatography. α‐T, γ‐T, α‐T3, and γ‐T3 were detected in all samples. The total concentration of tocopherol and tocotrienol was in the range of 4.8–9.9 mg/100 g seed (35.3–68.8 mg/100 g oil basis). The Muscat Bailey A cultivar had the highest total tocopherol and tocotrienol contents, followed by Canner and Naples. γ‐T3 ranged from 1.6 to 4.9 mg/100 g seed (11.2 to 53.81 mg/100 g oil basis) and was the main isomer, followed by α‐T3 in most of the samples. Analytical method validation parameters including accuracy and precision were determined. Overall recovery from grape seeds was close to 100%.