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Rapid and efficient generation of T‐cell receptor‐like antibodies using chip‐based single‐cell analysis
Author(s) -
Ozawa Tatsuhiko,
Kobayashi Eiji,
Hamana Hiroshi,
Nakamura Tomoko,
Lyu Fulian,
Hayashi Atsushi,
Muraguchi Atsushi,
Kishi Hiroyuki
Publication year - 2021
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.202049083
Subject(s) - biology , antibody , microbiology and biotechnology , computational biology , cell , chip , immunology , genetics , computer science , telecommunications
Generation of TCR‐like monoclonal antibodies using conventional methods is markedly laborious and inefficient. We have proposed improvements of ISAAC (chip‐based Ab‐secreting cell [ASC] screening method), allows comprehensive analysis of ASCs at the single‐cell level to obtain TCR‐like antibodies; blocking procedure enables us to avoid the detection of non‐TCR‐like antibodies.