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Characteristics of the T‐dependent α(1 → 6) glucosyl (dextran) antibody response induced in mice with isomaltohexaose coupled to chicken γ‐globulin
Author(s) -
Moreno Carlos,
Hale Christine,
Hewett Rosemary,
Cussell Deborah
Publication year - 1979
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830091116
Subject(s) - biology , isoelectric focusing , antibody , microbiology and biotechnology , immunology , dextran , adjuvant , bordetella pertussis , biochemistry , enzyme , genetics , bacteria
Abstract Isomaltohexaose flavazole coupled to chicken γ‐globulin (IM6‐CGG) induced T celldependent anti‐α( 1 → 6) dextran‐specific IgM and IgG responses in CBA, BALB/c and A strain mice. The IgG responses were of restricted heterogeneity as judged by isoelectric focusing, and belonged mostly to the IgG 1 subclass with a minor IgG 3 component in the case of BALB/c and CBA mice. All four subclasses of IgG were produced in A strain mice. In contrast, native dextran B 512 induces exclusively T cell‐independent IgM responses of the same specificity for all 3 strains. All BALB/c mice immunized with different doses of IM6‐CGG either in Freund's adjuvant or with A1(OH) 3 plus Bordetella pertussis showed the same spectrotypes by isoelectric focusing. Sera obtained at different times after immunization of BALB/c mice failed to show spectrotype variations. Late, but not early, bleedings from CBA mice showed a tendency towards more uniform isoelectric focusing patterns.