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A circulatory transcriptional regulation among daf‐9, daf‐12 , and daf‐16 mediates larval development upon cholesterol starvation in Caenorhabditis elegans †
Author(s) -
Jeong MyungHwan,
Kawasaki Ichiro,
Shim YhongHee
Publication year - 2010
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.22322
Subject(s) - biology , decay accelerating factor , caenorhabditis elegans , mutant , microbiology and biotechnology , rna interference , transcription factor , downregulation and upregulation , gene , genetics , rna , immune system , complement system
C. elegans shows dauer‐like larvae formation upon cholesterol starvation (CS), but the genetic epistasis among abnormal da uer f ormation ( daf ) genes during the process remains unclear. To clarify the genetic interactions among daf‐9, daf‐12 , and daf‐16 in this process, mRNA levels of these genes upon CS were measured. CS increased the mRNA levels of daf‐9, daf‐12 , and daf‐16 . CS also induced DAF‐16 nuclear localization, which was positively and negatively regulated by DAF‐12 and DAF‐9 activities, respectively. Activated DAF‐16, a FOXO transcription factor, enhanced daf‐12 but suppressed daf‐9 expression, whereas DAF‐9 inhibited daf‐12 expression. Concomitantly, CS‐induced larval arrest was regulated positively by DAF‐12 and DAF‐16, but negatively by DAF‐9. The larval arrest in daf‐9 mutant was suppressed by daf‐12 RNAi, placing DAF‐12 downstream of DAF‐9. These results altogether suggest that circulatory mutual regulation among daf‐9, daf‐12 , and daf‐16 at the expression level mediates cholesterol signal to control larval development upon CS. Developmental Dynamics 239:1931–1940, 2010. © 2010 Wiley‐Liss, Inc.
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