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Enhanced sensitivity and stability in two‐color in situ hybridization by means of a novel chromagenic substrate combination
Author(s) -
Hurtado Romulo,
Mikawa Takashi
Publication year - 2006
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.20909
Subject(s) - biology , in situ hybridization , in situ , substrate (aquarium) , sensitivity (control systems) , stability (learning theory) , biophysics , anatomy , genetics , computer science , physics , ecology , engineering , machine learning , gene expression , electronic engineering , gene , meteorology
Abstract Double in situ hybridization analysis is a fundamental technique for studying the expression of two genes with high temporal and spatial resolution. However, due to the lack of sensitivity in current detection methods, this approach is powerful only when at least one transcript is abundantly expressed. Here, we report a new enzyme/chromagenic substrate combination that provides sufficient sensitivity for detecting two less abundant transcripts and stability for subsequent paraffin sectioning. Developmental Dynamics 235:2811–2816, 2006. © 2006 Wiley‐Liss, Inc.