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CD133 expression in circulating hematopoietic progenitor cells
Author(s) -
Cimato Thomas R.,
Conway Alexis,
Nichols Julianne,
Wallace Paul K.
Publication year - 2019
Publication title -
cytometry part b: clinical cytometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 61
eISSN - 1552-4957
pISSN - 1552-4949
DOI - 10.1002/cyto.b.21732
Subject(s) - haematopoiesis , progenitor cell , microbiology and biotechnology , expression (computer science) , progenitor , biology , stem cell , computer science , programming language
Background Circulating hematopoietic progenitors (HPCs) are involved in inflammatory diseases such as atherosclerosis, the immune response to cancer, and disorders of the hematopoietic system. HPC characterization by flow cytometry typically utilizes CD34 in combination with other cell surface markers to identify cell populations that give rise to specific hematopoietic lineages. CD133, also known as prominin‐1, is a cell surface protein found in HPCs that has a similar but not interchangeable expression pattern with CD34 for characterization of HPC populations. Our goal was to determine the distribution of CD133 expression within HPC sub‐types amongst circulating CD34+ cells. Methods The quantity of different HPC populations within the CD34+ CD133+ and CD34+ CD133− fractions of venous blood obtained from healthy human subjects was measured using multicolor flow cytometry. Results The majority of circulating CD34+ cells is CD133−. CD133+ CD34+ cells express low levels of CD38, contain cell populations bearing cell surface markers of hematopoietic stem cells, multipotent progenitors, and multilymphoid progenitors, and are largely devoid of CD38 expressing lineage specified progenitors. These findings clarify the composition of circulating CD133+ CD34+ cell types in adult human subjects. © 2018 International Clinical Cytometry Society