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High density lipoprotein modulates platelet function
Author(s) -
Barlage Stefan,
Boettcher Dorothee,
Boettcher Alfred,
Dada Ashraf,
Schmitz Gerd
Publication year - 2006
Publication title -
cytometry part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 90
eISSN - 1552-4930
pISSN - 1552-4922
DOI - 10.1002/cyto.a.20214
Subject(s) - abca1 , platelet , platelet activation , western blot , high density lipoprotein , lipoprotein , lipid raft , reverse cholesterol transport , cholesterol , flow cytometry , chemistry , receptor , biology , medicine , endocrinology , microbiology and biotechnology , biochemistry , transporter , immunology , gene
Abstract Background Platelet activation by atherogenic lipoproteins can be antagonized by high density lipoprotein (HDL), probably via interaction with the ATP‐binding cassette transporter A1 (ABCA1). Methods ABCA1 expression and its association with cholesterol rich membrane domains was analyzed by mRNA and Western blot analysis. HDL effects on platelet receptor clustering were analyzed by flow cytometric analysis of fluorescence resonance energy transfer between fluorochrome‐labeled antibodies. Results ABCA1 expression increased upon megakaryocytic differentiation of human stem cells and ABCA1 protein partially associated to LubroIWX‐resistant membrane domains. Plasma HDL‐cholesterol in healthy donors negatively correlated to the platelet membrane cholesterol content. Receptor cluster analysis revealed a decrease in the association of Gplb and FcγRII upon incubation of platelets with HDL3. Conclusion Our results suggest that HDL modulates platelet reactivity by altering lipid raft associated receptor clustering. © 2006 International Society for Analytical Cytology