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A Novel Method of Cellular Labeling: Anchoring MR‐Imaging Reporter Particles on the Outer Cell Surface
Author(s) -
Gianolio Eliana,
Giovenzana Giovanni Battista,
Ciampa Anna,
Lanzardo Stefania,
Imperio Daniela,
Aime Silvio
Publication year - 2008
Publication title -
chemmedchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.817
H-Index - 100
eISSN - 1860-7187
pISSN - 1860-7179
DOI - 10.1002/cmdc.200700182
Subject(s) - anchoring , confocal microscopy , nanoparticle , biophysics , membrane , cell membrane , cell , confocal , chemistry , linker , microscopy , materials science , nanotechnology , microbiology and biotechnology , biology , pathology , biochemistry , computer science , optics , medicine , physics , structural engineering , engineering , operating system
Cellular labeling . MR visualization of cells has been attained by anchoring negatively charged Gd‐loaded nanoparticles at the cellular membrane by a polycationic linker. Each nanoparticle contains approximately 800 Gd‐chelates and each cell has been loaded with approximately 5×10 6 particles. In this way only 15 % of the negative charges on the membrane has been used. The method has been validated by confocal microscopy.