Premium
Detection of Tumor Necrosis Factor Gene by Piezoelectric Nucleic Acid Sensor
Author(s) -
Hu Yi,
Zhou XtaoDong,
Liu LiJia,
Hu Min,
Pei RenJun,
Hu JiMing
Publication year - 2002
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.20020201022
Subject(s) - nucleic acid , chemistry , glutaraldehyde , gene , tumor necrosis factor alpha , microbiology and biotechnology , biochemistry , chromatography , biology , endocrinology
Abstract A piezoelectric nucleic acid sensor was constructed for detection of tumor necrosis factor gene. Two methods were employed for immobilization of nucleic add probe on gold electrode of piezoelectric crystal. The results show that polyethyleneimine adhesion and glutaraldehyde cross‐linking method has higher sensitivity, stability and selectivity than protein A method. The solid‐phase nucleic acid hybridization of oligo nucleotides and tumor necrosis factor target gene sequence were monitored using this sensor. Tumor necrosis factor gene sequence (580 bp) was detected by mis nucleic acid sensor for the first time.