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Back Cover: Luminescent Metal–Organic‐Framework‐Based Label‐Free Assay of Polyphenol Oxidase with Fluorescent Scan (Chem. Eur. J. 27/2017)
Author(s) -
Li Yue,
Guo An,
Chang Lan,
Li WenJuan,
Ruan WenJuan
Publication year - 2017
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201700764
Subject(s) - polyphenol oxidase , fluorescence , luminescence , chemistry , substrate (aquarium) , polyphenol , combinatorial chemistry , enzyme , kinetics , photochemistry , oxidase test , biochemistry , materials science , optoelectronics , antioxidant , biology , peroxidase , physics , ecology , quantum mechanics
A MOF‐based label‐free enzyme assay was designed in this work. To analyze polyphenol oxidase (PPO) activity, a luminescent MOF in which the emission could be selectively quenched by the o ‐quinone product was synthesized. With it, the kinetics of PPO‐catalyzed oxidation could be reflected with the fluorescence signal. This method exhibits the advantages of high sensitivity, wide substrate adaptability, and minimal effects on the enzymatic reaction. More information can be found in the Full Paper by W.‐J. Ruan, et al. on page 6562 ff.