Premium
An Autoimmolative Spacer Allows First‐Time Incorporation of a Unique Solid‐State Fluorophore into a Detection Probe for Acyl Hydrolases
Author(s) -
Zhang Xiaobing,
Waibel Michael,
Hasserodt Jens
Publication year - 2010
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.200902412
Subject(s) - fluorophore , solid state , chemistry , state (computer science) , computer science , enzyme , combinatorial chemistry , stereochemistry , biochemistry , fluorescence , programming language , physics , optics
Mind your HPQs : A probe to detect specific peptidases based on the unique solid‐state fluorophore HPQ has been developed by the use of an autoimmolative cyclization spacer in conjunction with an acylated O,O‐acetal linked to HPQ (see scheme). Under our assay conditions this probe detected the enzyme with a minimum half‐time of only 560 s.