Premium
Skin‐derived precursors possess the ability of differentiation into the epidermal progeny and accelerate burn wound healing
Author(s) -
Bayati Vahid,
Abbaspour Mohammad Reza,
Neisi Niloofar,
Hashemitabar Mahmoud
Publication year - 2017
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10717
Subject(s) - keratinocyte , dermis , microbiology and biotechnology , wound healing , skin repair , epidermis (zoology) , stem cell , cellular differentiation , biology , skin equivalent , cell culture , immunology , anatomy , biochemistry , genetics , gene
Abstract Skin‐derived precursors (SKPs) are remnants of the embryonic neural crest stem cells that reside in the dermis until adulthood. Although they possess a wide range of differentiation potentials, their differentiation into keratinocyte‐like cells and their roles in skin wound healing are obscure. The present study aimed to investigate the differentiation of SKPs into keratinocyte‐like cells and evaluate their role in healing of third degree burn wounds. To this aim, SKPs were differentiated into keratinocyte‐like cells on tissue culture plate and collagen–chitosan scaffold prepared by freeze‐drying. Their differentiation capability was detected by real‐time RT‐PCR and immunofluorescence. Thereafter, they were cultured on scaffold and implanted in a rat model of burn wound. Histopathological and immunohistochemical analyses were employed to examine the reconstituted skin. The research findings revealed that SKPs were able to differentiate along the epidermal lineage and this ability can be enhanced on a suitable scaffold. Additionally, the results indicated that SKPs apparently promoted wound healing process and accelerate its transition from proliferating stage to maturational phase, especially if they were differentiated into keratinocyte‐like cells. Regarding the results, it is concluded that SKPs are able to differentiate into keratinocyte‐like cells, particularly when they are cultured on collagen–chitosan scaffold. Moreover, they can regenerate epidermal and dermal layers including thick collagen bundles, possibly through differentiation into keratinocyte‐like cells.