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Antibody‐Free Detection of Protein Tyrosine Nitration in Tissue Sections
Author(s) -
Wisastra Rosalina,
Poelstra Klaas,
Bischoff Rainer,
Maarsingh Harm,
Haisma Hidde J.,
Dekker Frank J.
Publication year - 2011
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201100148
Subject(s) - fluorophore , nitrotyrosine , tyrosine , western blot , biochemistry , chemistry , biomarker , computational biology , nitration , covalent bond , fluorescence , microbiology and biotechnology , biology , gene , organic chemistry , enzyme , nitric oxide synthase , physics , quantum mechanics
Inflamed proteins: Tyrosine nitration is a covalent post‐translational protein modification that represents a biomarker for inflammatory diseases. We introduce a novel method to detect nitrotyrosine in biological samples by chemoselective conversion into a fluorophore. We describe the applicability of this methodology to detect protein‐bound nitrotyrosine by fluorescence microscopy in tissue sections and on Western blot membranes.