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Cationic Polymers with Inhibition Ability of DNA Condensation Elevate Gene Expression
Author(s) -
Harada Atsushi,
Kimura Yuka,
Kono Kenji
Publication year - 2010
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201000394
Subject(s) - plasmid , gene expression , dna , cationic polymerization , dna condensation , chemistry , transfection , gene delivery , gene , microbiology and biotechnology , biophysics , biochemistry , biology , polymer chemistry
Plasmid DNA molecules in polyplexes with poly( L ‐lysine) (PLL) and multiarm PEG‐installed PLL ( ma PEG–PLL) adopted condensed and noncondensed states, respectively. ma PEG–PLL polyplexes showed comparable cell‐free gene expression with naked pDNA. The inhibition ability of DNA condensation of ma PEG–PLL provided not only the recovery of cell‐free gene expression but also elevated the gene expression of cultured cells.