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Generation of Site‐Specific and Enzymatically Stable Conjugates of Recombinant Proteins with Ubiquitin‐Like Modifiers by the Cu I ‐Catalyzed Azide–Alkyne Cycloaddition
Author(s) -
Weikart Nadine D.,
Mootz Henning D.
Publication year - 2010
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200900738
Subject(s) - sumo protein , ubiquitin , recombinant dna , conjugate , click chemistry , azide , chemistry , cycloaddition , protein tag , combinatorial chemistry , alkyne , chemical biology , computational biology , biochemistry , catalysis , biology , fusion protein , organic chemistry , gene , mathematical analysis , mathematics
Click here for SUMOylation: Post‐translational modifications with ubiquitin and ubiquitin‐like proteins as SUMO are implicated in many biological processes. However, the preparation of defined conjugates by enzymatic means is often impossible. We report a new chemical‐conjugation approach to specifically and stably link these post‐translational modifiers to recombinant substrate proteins.