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A Simple Method for Preparing Peptide C‐Terminal Thioacids and Their Application in Sequential Chemoenzymatic Ligation
Author(s) -
Tan XiaoHong,
Zhang Xiaohong,
Yang Renliang,
Liu ChuanFa
Publication year - 2008
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200700740
Subject(s) - ligation , chemical ligation , peptide , native chemical ligation , combinatorial chemistry , chemistry , computer science , biochemistry , cysteine , microbiology and biotechnology , enzyme , biology
A sequential chemoenzymatic peptide ligation scheme is demonstrated by using consecutive mini‐thiol capture ligation and subtiligase‐catalyzed ligation in alternate steps. The key peptide thioacid building blocks are prepared easily by hydrothiolysis of peptide thioesters.
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