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Catalysis and Rational Engineering of trans‐Acting pH6DZ1, an RNA‐Cleaving and Fluorescence‐Signaling Deoxyribozyme with a Four‐Way Junction Structure
Author(s) -
Shen Yutu,
Chiuman William,
Brennan John D.,
Li Yingfu
Publication year - 2006
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200600195
Subject(s) - deoxyribozyme , fluorescence , chemistry , fluorophore , dna , rational design , moiety , catalysis , rna , g quadruplex , biosensor , substrate (aquarium) , biophysics , photochemistry , stereochemistry , combinatorial chemistry , biochemistry , nanotechnology , biology , gene , materials science , physics , quantum mechanics , ecology
Biosensor made of a catalytic DNA . When the RNA moiety (red) in the fluorogenic DNA substrate (purple) is cleaved by the bound catalytic DNA (blue) with a multiduplex structure, a strong fluorescence signal is generated owing to the separation of the fluorophore (F) from the quencher (Q).