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Stimulation of human platelets with concanavalin a involves phospholipase C activation
Author(s) -
Torti Mauro,
Balduini Cesare,
Ramaschi Giuseppe,
Sinigaglia Fabiola
Publication year - 1992
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.290100109
Subject(s) - concanavalin a , egta , cytochalasin b , antiporter , phospholipase c , leupeptin , chemistry , biochemistry , cytochalasin , gtp' , phospholipase , biophysics , calcium , cytoskeleton , microbiology and biotechnology , membrane , biology , enzyme , protease , organic chemistry , cell , in vitro
Abstract In response to concanavalin A, cytoplasmic calcium movement was observed in human platelets, both in the presence of 1 mM Ca 2+ or 1 mM EGTA in the medium. Concanavalin A also caused the activation of inositide turnover and the production of inositol phosphates, suggesting that activation of phospholipase C occurs. The mechanism by which concanavalin A stimulates phospholipase C does not depend on GTP‐binding transducers, because it was not inhibited by GDPβS, while experiments performed in the presence of cytochalasin B suggested a role for membrane glycoprotein IIb‐IIIa‐cytoskeleton interaction in this process. Ca 2+ ‐proteases and Na + /H + antiport also seemed to be related to concanavalin A‐induced phospholipase C activation, as suggested by experiments performed in the presence of leupeptin and amiloride.