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Bioactive Triterpenoids from Kadsura heteroclita
Author(s) -
Xu LiJia,
Peng ZongGen,
Chen HongShan,
Wang Jie,
Xiao PeiGen
Publication year - 2010
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.200900173
Subject(s) - chemistry , phytochemical , stereochemistry , cytotoxic t cell , in vitro , hydrobromide , two dimensional nuclear magnetic resonance spectroscopy , triterpenoid , cell culture , traditional medicine , biochemistry , organic chemistry , biology , medicine , genetics
Abstract A phytochemical study of Kadsura heteroclita led to the isolation of eight triterpenoids, including two new compounds, named kadheterilactone A ( 1 ) and kadheterilactone B ( 2 ), as well as six known compounds, longipedlactone H ( 3 ), longipedlactone A ( 4 ), longipedlactone F ( 5 ), kadsuranic acid A ( 6 ), nigranoic acid ( 7 ), and schisandronic acid ( 8 ). Their structures were elucidated on the basis of spectroscopic methods, including 2D NMR techniques. The cytotoxic activities of 1 – 8 were tested against several tumor cell lines by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl‐2 H ‐tetrazolium hydrobromide (MTT) assay in vitro. As a result, 4 and 5 turned out to be significantly cytotoxic against Hep‐G2 and Bel‐7402 tumor cell lines. All compounds were also tested for inhibition on HIV‐1 protease (PR) and reverse transcriptase (RT). Compounds 6 and 7 showed strong inhibition on HIV‐1 PR, while 8 exhibited moderate activity, others were only weakly active. No compounds were active against HIV‐1 RT.