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Neurite outgrowth of PC12 cells on diX (parylene) family materials
Author(s) -
Hoshino Takayuki,
Saito Itsuro,
Takano Haruka,
Samejima Kazuyuki,
Mabuchi Kunihiko,
Kato Yasuhiro X.
Publication year - 2011
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.739
Subject(s) - neurite , biocompatibility , cell culture , microbiology and biotechnology , cellular differentiation , chemistry , in vitro , cell , neuron , coating , biophysics , materials science , biomedical engineering , nanotechnology , biology , neuroscience , biochemistry , medicine , organic chemistry , gene , genetics
Abstract We investigated neuronal cell differentiation, particularly neurite outgrowth, on the surface of diX H and diX AM using an in vitro examination of a neuron‐like rat pheochromocytoma cell line, PC12. diX H and diX AM are in the parylene family of diX C (or Parylene‐C), which is widely used as a novel coating material to insulate neural electrodes, and they have been recently commercialized; diX H and diX AM offer different features of biocompatibility. Previously, we found that these new parylene materials have high cell adhesiveness to neuronal cells whereas the adhesiveness of diX C is extremely low. However, their cell differentiation remains unknown although neuronal cell differentiation plays a crucial role in their development and regeneration. This study showed that almost all PC12 cells adhering to the surface of diX AM and diX H were differentiated, but the neurite outgrowth was significantly larger on diX H than that on diX AM and a conventional polystyrene culture dish. The result suggests that diX H may be advantageous as a biocompatible coating material for a scaffold, which can be used on virtually any substrate to get various configurations in neural devices. © 2011 American Institute of Chemical Engineers Biotechnol. Prog., 2012