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Enhancement of biomolecule transport by electroporation: A review of theory and practical application to transformation of Corynebacterium glutamicum
Author(s) -
Tryfona Theodora,
Bustard Mark T.
Publication year - 2006
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.20725
Subject(s) - corynebacterium glutamicum , electroporation , membrane permeability , biomolecule , transformation (genetics) , membrane , cell permeability , biochemical engineering , chemistry , biophysics , nanotechnology , computational biology , biochemistry , biology , materials science , engineering , gene
Abstract Selective and reversible permeabilization of the cell wall permeability barrier is the focus for many biotechnological applications. In this article, the basic principles for reversible membrane permeabilization, based on biological, chemical, and physical methods are reviewed. Emphasis is given to electroporation (electropermeabilization) which tends to be the most popular method for membrane permeabilization and for introduction of foreign molecules into the cells. The applications of this method in industrial processes as well as the critical factors and parameters which affect the success of this approach are discussed. The different strategies developed throughout the years for increased transformation efficiencies of the industrially important amino acid‐overproducing bacterium Corynebacterium glutamicum , are also summarized. © 2005 Wiley Periodicals, Inc.