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Dispersion equation and polarizability of bovine serum albumin from measurements of refractive indices
Author(s) -
Andersen M.,
Painter L. R.,
Nir S.
Publication year - 1974
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1974.360130616
Subject(s) - polarizability , bovine serum albumin , chemistry , refractive index , dispersion (optics) , analytical chemistry (journal) , chromatography , solvent , serum albumin , optics , organic chemistry , biochemistry , molecule , physics
Refractive indices of bovine serum albumin solutions in saline and in 2‐chloro ethanol are measured for the spectral region 1770–10000 Å by a reflectance method. A procedure for the determination of a dispersion formula for binary mixtures is developed and applied. The electronic polarizability of bovine serum albumin is determined and is found to be almost independent of the solvent and concentration. Hence it follows that the electronic polarizability of bovine serum albumin is almost independent of the molecular environment.