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Chemiluminescence enzyme immunoassay (CLEIA) for the determination of chloramphenicol residues in aquatic tissues
Author(s) -
Chuanlai Xu,
Cifang Peng,
Kai Hao,
Zhengyu Jin,
Wukang Wang
Publication year - 2006
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.892
Subject(s) - chemiluminescence , chromatography , chemistry , detection limit , shrimp , immunoassay , chloramphenicol , linear range , coefficient of variation , biochemistry , antibiotics , biology , fishery , antibody , immunology
Abstract A competitive indirect chemiluminescence enzyme immunoassay (ic‐CLEIA) for chloramphenicol (CAP) residues in shrimp has been developed. After optimization (incubation time, concentration of Tween‐20, concentration of PBS and pH), the method gave a limit of detection of 0.01 ng/mL and a detection range of 0.03–23.7 ng/mL, with an ED 50 of 0.47 ng/mL. The method has been validated on spiked shrimp samples in terms of precision (intra‐ and interassay coefficient variations of less than 10% and 15%, respectively) and accuracy (mean recovery 95–123%). The assay performance is better than the ELISA method which is widely used to detect chloramphenicol and indicates that the CLEIA method can be used to test aquatic samples instead of ELISA. Copyright © 2006 John Wiley & Sons, Ltd.