Investigations on vinylene carbonate. VI. Immobilization of alkaline phosphatase onto poly(vinylene carbonate) ‐jeffamine® hydrogel beads | Zendy

Chen Guohua | Zendy; Does Leen Van Der | Zendy; Bantjes Adriaan | Zendy

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Investigations on vinylene carbonate. VI. Immobilization of alkaline phosphatase onto poly(vinylene carbonate) ‐jeffamine® hydrogel beads

Author(s) -

Chen Guohua,

Does Leen Van Der,

Bantjes Adriaan

Publication year - 1993

Publication title -

journal of applied polymer science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.575

H-Index - 166

eISSN - 1097-4628

pISSN - 0021-8995

DOI - 10.1002/app.1993.070480707

Subject(s) - alkaline phosphatase , carbonate , chemistry , thermal stability , polymer , polymer chemistry , nuclear chemistry , enzyme , organic chemistry

Hydrogel‐like polymers with reactive cyclic carbonate (CCA) groups have been obtained by the cross‐linking reaction of poly(vinylene carbonate) (PVCA) with Jeffamines®. By this reaction, microspheric hydrogel beads with a high water content and a high concentration of reactive CCA groups were prepared. The beads were used as a matrix for the immobilization of the enzyme alkaline phosphatase (ALP) and showed a considerable capacity to couple ALP and a reasonable retention of activity for the immobilized ALP, depending on the reaction conditions. The immobilized ALP exhibited a better thermal stability than did native ALP and a high residual activity was found after repeated use. © 1993 John Wiley & Sons, Inc.

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