Premium
Infrared Based Saliva Screening Test for COVID‐19
Author(s) -
Wood Bayden R.,
Kochan Kamila,
Bedolla Diana E.,
SalazarQuiroz Natalia,
Grimley Samantha L.,
PerezGuaita David,
Baker Matthew J.,
Vongsvivut Jitraporn,
Tobin Mark J.,
Bambery Keith R.,
Christensen Dale,
Pasricha Shivani,
Eden Anthony K.,
Mclean Aaron,
Roy Supti,
Roberts Jason A.,
Druce Julian,
Williamson Deborah A.,
McAuley Julie,
Catton Mike,
Purcell Damian F. J.,
Godfrey Dale I.,
Heraud Philip
Publication year - 2021
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.202104453
Subject(s) - covid-19 , saliva , test (biology) , virology , medicine , psychology , biology , outbreak , disease , infectious disease (medical specialty) , paleontology
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) has resulted in an unprecedented need for diagnostic testing that is critical in controlling the spread of COVID‐19. We propose a portable infrared spectrometer with purpose‐built transflection accessory for rapid point‐of‐care detection of COVID‐19 markers in saliva. Initially, purified virion particles were characterized with Raman spectroscopy, synchrotron infrared (IR) and AFM‐IR. A data set comprising 171 transflection infrared spectra from 29 subjects testing positive for SARS‐CoV‐2 by RT‐qPCR and 28 testing negative, was modeled using Monte Carlo Double Cross Validation with 50 randomized test and model sets. The testing sensitivity was 93 % (27/29) with a specificity of 82 % (23/28) that included positive samples on the limit of detection for RT‐qPCR. Herein, we demonstrate a proof‐of‐concept high throughput infrared COVID‐19 test that is rapid, inexpensive, portable and utilizes sample self‐collection thus minimizing the risk to healthcare workers and ideally suited to mass screening.