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Signal Amplification by Glyco‐qPCR for Ultrasensitive Detection of Carbohydrates: Applications in Glycobiology
Author(s) -
Kwon Seok Joon,
Lee Kyung Bok,
Solakyildirim Kemal,
Masuko Sayaka,
Ly Mellisa,
Zhang Fuming,
Li Lingyun,
Dordick Jonathan S.,
Linhardt Robert J.
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201205112
Subject(s) - glycobiology , chemistry , computational biology , carbohydrate , biochemistry , biology , glycan , glycoprotein
Tiny amounts of carbohydrates (ca. 1 zmol) can be detected quantitatively by a real‐time method based on the conjugation of carbohydrates with DNA markers (see picture). The proposed method (glyco‐qPCR) provides uniform, ultrasensitive detection of carbohydrates, which can be applied to glycobiology, as well as carbohydrate‐based drug discovery.