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Cryo‐ EM structure of the Min CD copolymeric filament from Pseudomonas aeruginosa at 3.1 Å resolution
Author(s) -
SzewczakHarris Andrzej,
Wagstaff James,
Löwe Jan
Publication year - 2019
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.13471
Subject(s) - protein filament , pseudomonas aeruginosa , chemistry , microbiology and biotechnology , bacteria , biology , biochemistry , genetics
Positioning of the division site in many bacterial species relies on the Min CDE system, which prevents the cytokinetic Z‐ring from assembling anywhere but the mid‐cell, through an oscillatory diffusion‐reaction mechanism. MinD dimers bind to membranes and, via their partner MinC, inhibit the polymerization of cell division protein FtsZ into the Z‐ring. MinC and MinD form polymeric assemblies in solution and on cell membranes. Here, we report the high‐resolution cryo‐ EM structure of the copolymeric filaments of Pseudomonas aeruginosa Min CD . The filaments consist of three protofilaments made of alternating MinC and MinD dimers. The Min CD protofilaments are almost completely straight and assemble as single protofilaments on lipid membranes, which we also visualized by cryo‐ EM .