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Tat transport in Escherichia coli requires zwitterionic phosphatidylethanolamine but no specific negatively charged phospholipid
Author(s) -
Rathmann Claudia,
Schlösser Amelie S.,
Schiller Jürgen,
Bogdanov Mikhail,
Brüser Thomas
Publication year - 2017
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12794
Subject(s) - phosphatidylethanolamine , cardiolipin , phosphatidylglycerol , phosphatidic acid , escherichia coli , phospholipid , chemistry , biochemistry , diacylglycerol kinase , phosphatidylcholine , strain (injury) , biology , membrane , enzyme , gene , protein kinase c , anatomy
Translocation of folded proteins by the Tat system of Escherichia coli is believed to rely on the presence of phosphatidylethanolamine ( PE ) and the negatively charged phospholipids cardiolipin ( CL ) and phosphatidylglycerol ( PG ). Here, we show that while PE is indeed essential for activity, the Tat system is fully functional in a clsA / clsB / clsC deletion strain lacking CL , and in a pgsA deletion strain lacking both PG and CL during aerobic growth on complex media. In contrast to early studies that relied on strains with reduced lipid levels, this study therefore demonstrates that PG and CL are dispensable for Tat transport. The lack of these lipids may be compensated by other anionic phospholipids such as phosphatidic acid, CDP ‐diacylglycerol or N‐acyl‐ PE .