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Thioesterase overexpression in Nicotiana benthamiana leaf increases the fatty acid flux into triacylgycerol
Author(s) -
El Tahchy Anna,
Reynolds Kyle B.,
Petrie James R.,
Singh Surinder P.,
Vanhercke Thomas
Publication year - 2017
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12539
Subject(s) - nicotiana benthamiana , arabidopsis thaliana , transgene , oleosin , chloroplast , arabidopsis , nicotiana , biochemistry , fatty acid , botany , thioesterase , chemistry , biology , microbiology and biotechnology , biosynthesis , gene , solanaceae , mutant
Increasing the oil content of leafy biomass is emerging as a sustainable source of vegetable oil to meet global demand. Transient gene expression in leaf provides a reproducible platform to study the effect of transgenes on lipid biosynthesis. We first generated a transgenic Nicotiana benthamiana line containing high levels of triacylglycerol in the leaf tissue (31.4% by dry weight) by stably expressing WRI 1 , DGAT 1 and OLEOSIN . We then used this line as a platform to test the effect of three Arabidopsis thaliana thioesterases ( FATA 1, FATA 2 and FATB ). Further increases in leaf oil content were observed with biochemical and lipid assays revealing an increase in the export of fatty acids from the chloroplast and a modification in the oil profile.