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A flow injection chemiluminescence method using Cr(III) as a catalyst for determining hydrogen peroxide. Application to H 2 O 2 determination in cultures of microalgae
Author(s) -
Escobar R.,
GarcíaDomínguez S.,
Guiraúm A.,
Montes O.,
Galván F.,
de la Rosa F. F.
Publication year - 2000
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/1522-7243(200005/06)15:3<131::aid-bio554>3.0.co;2-8
Subject(s) - chemiluminescence , hydrogen peroxide , luminol , catalysis , horseradish peroxidase , flow injection analysis , chemistry , detection limit , peroxide , nuclear chemistry , chromatography , inorganic chemistry , organic chemistry , enzyme
Flow injection analysis has been applied to the determination of hydrogen peroxide produced by some different species of microalgae. The method is based on the luminol–H 2 O 2 chemiluminescence reaction using Cr(III) as a catalyst. Optimum experimental conditions for the method have been studied and trace amounts of hydrogen peroxide determined with detection limits of 4 10 −8 mol/L. The method using Cr(III) was compared with that using horseradish peroxidase as the catalyst. © 2000 John Wiley & Sons, Ltd.
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