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Ontogeny, distribution and function of CD38‐expressing B lymphocytes in mice
Author(s) -
DonísHernández Felipe Raúl,
Parkhouse R. Mike E.,
SantosArgumedo Leopoldo
Publication year - 2001
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/1521-4141(200104)31:4<1261::aid-immu1261>3.0.co;2-h
Subject(s) - cd38 , immunoglobulin d , biology , cd5 , bone marrow , b cell , cd40 , immunology , b 1 cell , microbiology and biotechnology , lymphocyte , antibody , stem cell , t cell , immune system , in vitro , cytotoxic t cell , antigen presenting cell , biochemistry , cd34
Abstract Analysis of expression of CD38, CD45R (B220), IgM and IgD on splenic B lymphocytes from mice of different ages demonstrated CD38 on both immature (B220 + , BCR – ) and mature (B220 + , BCR + ) B lymphocytes. Similarly, CD38 is expressed as early as B220 on the surface of progenitor B cells in the bone marrow. In spite of expressing of CD38 and IgM, neonatal B cells, in contrast to the adult, failed to proliferate to either anti‐CD38 or anti‐IgM cross‐linking when IL‐4 was present. They did, however, respond to LPS and anti‐CD40, and by 2 weeks of age they began to respond to anti‐CD38 and anti‐IgM, reaching adult B cell levels by 4 weeks. Although the distribution of CD38 on adult B cells from most different lymphoid compartments was broadly similar, significantly higher levels of CD38 were expressed on peritoneal B lymphocytes. A detailed analysis, using IgM / IgD ratio and staining with anti‐CD5 confirmed that B1 lymphocytes were expressinga high level of CD38. Interestingly, both immature B cells and peritoneal B1 lymphocytes were unresponsive to anti‐CD38. However, they were activated by LPS or anti‐CD40.