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Solid‐Phase Synthesis of Doubly Labeled Peptide Nucleic Acids as Probes for the Real‐Time Detection of Hybridization
Author(s) -
Seitz Oliver
Publication year - 2000
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/1521-3773(20000915)39:18<3249::aid-anie3249>3.0.co;2-m
Subject(s) - peptide nucleic acid , nucleic acid , chemistry , peptide , combinatorial chemistry , nucleic acid thermodynamics , fluorescence , dna , peptide synthesis , homogeneous , hybridization probe , molecular beacon , phase (matter) , oligonucleotide , biochemistry , organic chemistry , base sequence , physics , quantum mechanics , thermodynamics
A substantial fluorescence enhancement was observed when novel peptide nucleic acid (PNA) probes were hybridized with complementary DNA in homogeneous solution. For the synthesis of the doubly labeled PNA probes, a highly flexible strategy was developed (see scheme). All reactions, including the labeling steps, were performed on the solid phase. Bhoc=benzhydryloxycarbonyl; Boc= t BuOCO.